sheep anti brdu  (Novus Biologicals)

Journal: Bioengineering

Article Title: Development of an Eye Irritation Test Method Using an In-House Fabrication of a Reconstructed Human Cornea-like Epithelium Model for Eye Hazard Identification

doi: 10.3390/bioengineering11040302

Figure Lengend Snippet: ( a ) Monitoring the recovery of the iHCE-NY1 model using histological cross-sections. HE-stained tissue specimens of the iHCE-NY1 model’s incubation for 1- (post-incubation period (PIP) 1), 7-(PIP 7), 14-(PIP 14), and 21-days (PIP 21) post exposure to liquid chemicals are shown. At PIP 1, cells with enriched nuclei (yellow arrowheads) were observed, while viable cells (blue arrowheads) were observed from PIP 7. As the culture days increased to PIP 14 and PIP 21, more viable cells were observed. The gray dotted line indicates the position of the bottom of the iHCE-NY1 model. #6: γ-butyrolactone, #16: 2-methyl-1-pentanol, all bar indicates 50 µm. ( b ) Detection of respirating cells in the iHCE-NY1 model. Cells in S phase of the cell cycle that took up BrdU had cell nuclei revealed with red fluorescence. At post-incubation period (PIP) 1-day, few cells were in the S phase. From PIP 7-day onward, BrdU-positive S-phase cells were detected in all samples. #6: γ-butyrolactone, #16: 2-methyl-1-pentanol, all bar indicates 50 µm.

Article Snippet: Fixed and paraffin sections were prepared, and BrdU was detected using a sheep polyclonal anti-BrdU antibody (1:100, Capralogics, Hardwick, MA, USA) and an anti-sheep IgG antibody labeled with Alexa Fluor 594 (1:1000, Thermo Fisher Scientific Inc., Waltham, MA, USA).

Techniques: Staining, Incubation, Fluorescence